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Peuterey Homme Stripes Yd Corporation Noir

Peuterey Homme Stripes Yd Corporation Noir

Hydrogen peroxide (H2O2) has been proposed to act as a factor for endothelium-derived hyperpolarization (EDH) and EDH may act as a ‘back up’ system to compensate the loss of the NO pathway. Here, the mechanism of action of H2O2 in porcine isolated coronary arteries (PCAs) was investigated. Distal PCAs were mounted in a wire myograph and pre-contracted with U46619 (1 nM–50 μM), a thromboxane A2-mimetic or KCl (60 mM). Concentration–response curves to H2O2(1 μM–1 mM), bradykinin (0.01 nM–1 μM), sodium nitroprusside (SNP) (10 nM–10 μM), Woolrich Suisse verapamil (1 nM–10 μM), KCl (0–20 mM) or Ca2+-reintroduction (1 μM–10 mM) were constructed in the presence of various inhibitors. Activity of the Na+/K+-pump was measured through rubidium-uptake using atomic absorption spectrophotometry. H2O2 caused concentration-dependent vasorelaxations with a maximum relaxation (Rmax) Parka Woolrich Homme of 100 ± 16% (mean ± SEM), pEC50 = 4.18 ± 0.20 Veste Woolrich Femme (n = 4) which were significantly inhibited by PEG-catalase at 0.1–1.0 mM H2O2 (P < 0.05). 10 mM TEA significantly inhibited the relaxation up to 100 μM H2O2 (P < 0.05). 60 mM K+ and 500 nM ouabain significantly inhibited H2O2-induced vasorelaxation producing a relaxation of 40.8 ± 8.5% (n = 5) and 47.5 ± 8.6% (n = 6) respectively at 1 mM H2O2 (P < 0.0001). H2O2-induced vasorelaxation was unaffected by the removal of endothelium, inhibition of NO, cyclo-oxygenase, gap junctions, SKCa, IKCa, BKCa Kir, KV, KATP or cGMP. 100 μM H2O2 had no effects on the KCl-induced vasorelaxation or Ca2+-reintroduction contraction. 1 mM H2O2 inhibited both KCl-induced vasorelaxation and rubidium-uptake consistent with inhibition of the Na+/K+-pump activity. We have shown that the vascular actions of H2O2 are sensitive to ouabain and high concentrations of H2O2 are able to modulate the Na+/K+-pump. This may contribute towards its vascular actions.

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